Research Article
Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes
Table 1
Summary of growth parameters, CFD-predicted specific power inputs (
), and local shear stress (LSS) levels.
| [rpm] | [W m−3] | LSS [10−3 N m−2] | Total cell numbers on day 7 [107 cells] | Living cell density on day 7 [106 cells mL−1] | EFs [—] | and [h−1] and [h] |
| 25 | 0.21 | 3.21/69 | 8.1 ± 0.1 | 0.81 ± 0.01 | 71.4 ± 0.2 | 0.026 ± 0.001 26.7 ± 0.1 | 43 | 0.65 | 4.43/142 | 6.2 ± 0.6 | 0.62 ± 0.06 | 79.6 ± 3.2 | 0.022 ± 0.001 31.5 ± 0.2 | 49 | 0.80 | 4.96/187 | 12.5 ± 0.5 | 1.25 ± 0.05 | 117.0 ± 4.7 | 0.029 ± 0.001 23.7 ± 0.1 | 63 | 1.24 | 6.72/224 | 11.1 ± 0.6 | 1.11 ± 0.06 | 97.4 ± 3.7 | 0.028 ± 0.001 24.8 ± 0.2 | 90 | 2.24 | 10.22/325 | 3.7 ± 0.7 | 0.37 ± 0.07 | 28.5 ± 5.1 | 0.020 ± 0.002 34.4 ± 0.4 | 120 | 3.63 | 13.56/437 | 2.5 ± 0.2 | 0.25 ± 0.02 | 19.4 ± 1.3 | 0.017 ± 0.001 41.3 ± 0.1 |
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LSS; local shear stress given with volume-weighted mean/maximum values. LSS and were adapted from Kaiser et al. [27].
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