Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes

Table 1

Summary of growth parameters, CFD-predicted specific power inputs (), and local shear stress (LSS) levels.

[rpm]	[W m^−3]	LSS [10^−3 N m^−2]	Total cell numbers on day 7 [10^7 cells]	Living cell density on day 7 [10^6 cells mL^−1]	EFs [—]	and [h^−1] and [h]
25	0.21	3.21/69	8.1 ± 0.1	0.81 ± 0.01	71.4 ± 0.2	0.026 ± 0.001; 26.7 ± 0.1
43	0.65	4.43/142	6.2 ± 0.6	0.62 ± 0.06	79.6 ± 3.2	0.022 ± 0.001; 31.5 ± 0.2
49	0.80	4.96/187	12.5 ± 0.5	1.25 ± 0.05	117.0 ± 4.7	0.029 ± 0.001; 23.7 ± 0.1
63	1.24	6.72/224	11.1 ± 0.6	1.11 ± 0.06	97.4 ± 3.7	0.028 ± 0.001; 24.8 ± 0.2
90	2.24	10.22/325	3.7 ± 0.7	0.37 ± 0.07	28.5 ± 5.1	0.020 ± 0.002; 34.4 ± 0.4
120	3.63	13.56/437	2.5 ± 0.2	0.25 ± 0.02	19.4 ± 1.3	0.017 ± 0.001; 41.3 ± 0.1

LSS; local shear stress given with volume-weighted mean/maximum values. LSS and were adapted from Kaiser et al. [27].