Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes
Table 2
Specific metabolic consumption and production rates.
[rpm]
[pmol cell−1 d−1]
[pmol cell−1 d−1]
[mmol mmol−1]
[pmol cell−1 d−1]
[mmol mmol−1]
25
−1.86 ± 0.01
4.31 ± 0.03
2.87 ± 0.01
0.259 ± 0.004
2.65 ± 0.41
43
−2.30 ± 0.20
4.39 ± 0.30
1.94 ± 0.30
0.252 ± 0.003
2.66 ± 0.29
49
−1.58 ± 0.11
2.43 ± 0.17
1.55 ± 0.11
0.090 ± 0.001
2.46 ± 0.46
63
−1.08 ± 0.23
2.92 ± 1.04
2.51 ± 0.39
0.191 ± 0.052
2.36 ± 0.49
90
−4.09 ± 0.52
7.78 ± 1.39
1.89 ± 0.10
0.596 ± 0.133
2.43 ± 0.11
120
−5.07 ± 0.19
8.82 ± 0.11
1.74 ± 0.04
0.878 ± 0.085
2.57 ± 0.05
: specific glucose consumption rate; : specific lactate production rate; : specific ammonia production rate; : specific lactate yield per unit glucose; : specific ammonia yield per unit glutamine. ().