Research Article
Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes
Table 3
Summary of predicted shear stress levels and specific power inputs in the BIOSTAT CultiBag RM 2L.
| Working volume [L] | Rocking angle [°] | Rocking rate [rpm] | [W m−3] | MLSS [10−3 N m−2] |
| 0.5 | 4 | 26 | 20.61/40.66 | 5.83/664 | 0.5 | 6 | 22 | 32.52/53.55 | 5.44/509 | 0.5 | 6 | 35 | 156.01/262.60 | 5.78/597 | 0.5 | 8 | 18 | 47.71/85.79 | 29.61/704 | 0.5 | 10 | 14 | 93.40/149.73 | 20.61/885 | 0.5 | 10 | 25 | 144.10/259.76 | 26.40/3162 |
| 1.0 | 4 | 29 | 34.40/65.33 | 4.91/916 | 1.0 | 6 | 25 | 53.70/98.16 | 4.75/1194 | 1.0 | 8 | 20 | 32.14/53.35 | 1.09/289 | 1.0 | 10 | 15 | 81.10/123.77 | 5.68/1012 | 1.0 | 10 | 25 | 118.32/203.76 | 10.80/4042 |
| 1.5 | 4 | 31 | 8.92/17.69 | 0.49/214 | 1.5 | 6 | 27 | 17.96/32.45 | 0.60/256 | 1.5 | 8 | 22 | 26.56/50.04 | 0.74/279 | 1.5 | 10 | 16 | 68.85/105.28 | 3.15/909 | 1.5 | 10 | 25 | 95.02/155.46 | 4.86/2959 |
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: mean and maximum values of specific power input. MLSS: maximum values of local volume-weighted mean and maximum shear stresses over one period.
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