Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro

Figure 3

MSCs and the fd-ECM synergistically downregulate WHCO1 and MDA MB 231 gene expression. (a) Western blot analysis of lysates from WHCO1 cells cocultured with MSCs, cultured on an fd-ECM, or in combination, for 48 hrs showing expression of PCNA, Bcl-2, MMP-2, and p21. (b) Western blot analysis of lysates from MDA MB 231 cells cocultured with MSCs, cultured on an fd-ECM, or in combination, for 48 hrs showing expression of the same genes as in (a). (c) Immunofluorescence staining for Bcl-2 in WHCO1 cells after coculture with WHCO1 cells (second panel) and WJ-MSCs (third panel) and also cultured on fd-ECM (fourth panel). (d) Immunofluorescence staining for Bcl-2 in MDA MB 231 cells after coculture with MDA MB 231 cells (second panel) and WJ-MSCs (third panel) and also cultured on fd-ECM (fourth panel).

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