Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro

Figure 4

MSCs and the fd-ECM synergistically inhibit cancer cell proliferation. WHCO1 (a) and MDA MB 231 (b) cancer cells were cocultured with MSCs, cultured on fd-ECM, or in combination for the indicated time period. Control and treated cells were counted at the indicated times as described in Section 2. ((c)–(f)) Western blot analysis of cell lysates from control and treated WHCO1 and MDA MB 231 cells analyzed after 48 hrs of incubation using Ki67 antibody. Results obtained using MSCs from 2 donors are shown and are representative of all results obtained using WJ-MSCs from several other donors. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Experiments were performed in triplicate and repeated 3 times.

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