Research Article
NFATc4 Regulates Sox9 Gene Expression in Acinar Cell Plasticity and Pancreatic Cancer Initiation
Figure 4
Sox9 represents a transcriptional NFATc4 target in metaplastic pancreatic cells. (a)–(d) ChIP analyses in 266-6 cells ((a) and (c)) and in primary tumor cells from KrasG12D; ; pdx1-Cre mice ((b) and (d)) were conducted in the following EGF (3 hours) or TGF (24 hours) treatment, respectively. (a)-(b) qRT-PCR was performed to determine NFATc4 occupancy on the Sox9 promoter regions −825 and +370. Data was normalized to IgG and displayed as xfold binding compared to control. (c)-(d) H4K3me3 and polymerase II enrichment are demonstrated on the Sox9 promoter region +370. Data was normalized to IgG and displayed as xfold binding compared to control. (e)-(f) qRT-PCR to detect NFATc4 (e) and Sox9 (f) mRNA expressions following genetic depletion of NFATc4 and EGF treatment (3 hours).
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