Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells

Figure 3

TPM and SHG imaging of DAPI stained umbilical cord vein showing a differential staining intensity for DAPI. (a) AF signal and (b) SHG derived from the vessel media and adventitia. Increased AF density originates from the media smooth muscle cells. In addition, more SHG is observed in the media due to higher collagen content, as expected. (c) An increasing gradient in DAPI fluorescence was observed towards the media of the umbilical vein. (d) Merged image. Scale bar = 50 μm.

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