A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells

Figure 5

Validation of CellTiter-GLO assay on primary GSC cultures. The CellTiter-GLO assay was validated on primary glioma stem-like cell cultures. Cultures were treated with 50 μM (white bar) or 100 μM (black bars) TMZ (a), 3 Gy (white bars) or 6 Gy (black bars) radiation (b), MOI 1 (white bars), MOI 10 (grey bars), or MOI 100 (black bars) Delta-24-GFP-RGD (c), or 50, 100, 200, or 400 nM rapamycin (d). Read-out was after 5 days. Error bars indicate standard deviation, . Microscopic images of GS79 and GS295; nontreated (NT, left) and treated with 400 nM rapamycin (right) (e).

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