Bone Anabolic Effects of Soluble Si: In Vitro Studies with Human Mesenchymal Stem Cells and CD14+ Osteoclast Precursors

Figure 4

Effects of Si on the differentiation and function of CD14+ cells. (a) TRAP activity, determined by pNPP hydrolysis assay; (b) number of TRAP+ multinucleated cells, determined by TRAP and hematoxylin staining; (c) qPCR analysis of cell cultures maintained in the absence (control) or presence of (25 μM) Si; (d) calcium phosphate resorbing activity of cell cultures. Significantly different than the control.

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