Allogeneic Mesenchymal Stem Cell Treatment Induces Specific Alloantibodies in Horses

Figure 1

RBC crossmatch procedure to validate rabbit anti-equine polyclonal IgG antibody binding to equine cells. (a) Aa+ RBCs mixed with secondary antibody (no serum (primary antibody) control), (b) Aa+ RBCs mixed with serum from an Aa− horse that contains alloantibodies to Aa+ RBCs, (c) Aa− RBCs mixed with secondary antibody (no serum (primary antibody) control), and (d) Aa− RBCs mixed with serum from an Aa− horse that does not contain antibodies to Aa− RBCs.