Research Article
Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes
Table 1
Number of cells collected from colonies in subculture with fresh feeder cells.
| Passage | Number of collected cells from colonies | Number of used cells for subculture |
| 0 | 1.95 ± 0.03 × 106/12 wells | 2 × 104/well | 1 | 6.6 ± 0.9 × 105/12 wells | 2 × 104/well | 2 | 5.9 ± 0.6 × 105/12 wells | 2 × 104/well | 3 | 8.7 ± 0.9 × 105/12 wells | 2 × 104/well | 4 | 1.14 ± 0.12 × 106/12 wells | 2 × 104/well | 5 | 1.12 ± 0.11 × 106/12 wells | 2 × 104/well | 6 | 1.01 ± 0.26 × 106/12 wells | 2 × 104/well | 7 | 1.06 ± 0.12 × 106/12 wells | 2 × 104/well | 8 | 1.61 ± 0.36 × 106/12 wells | 2 × 104/well | 9 | 1.25 ± 0.2 × 106/12 wells | 2 × 104/well | 10 | 1.31 ± 0.58 × 106/12 wells | 2 × 104/well |
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