p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation

Figure 5

Impact of p53 status on oxygen effect of long-term in vitro culturing and colony formation capacity of mMSCs. (a) Morphology of mMSCs p53−/− cultured in hypoxic conditions (left image) and in normoxic conditions (right image). Representative images show mMSCs p53−/− cultured for 20 days. (b) Colony forming ability of mMSCs p53−/− after 14 days in hypoxic (grey bar) and normoxic (black bar) cultures calculated as mean values of fibroblast colony-forming units (CFU-F) per plated cell (3 biological replicates).

(a)

(b)