Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34⁺ Cells

Figure 5

The effects of MSC-MVs on the generation of CFCs and CAFCs and the expression of β-catenin in the expanded CD34⁺ cells. (a) Total number of CFCs generated in the four groups after 7 and 14 days of expansion (), compared with the control group, compared with the MSC group. (b) Typical morphology of BFU-E, CFU-GM, and CFU-GEMM, observed using an inverted microscope and after Wright-Giemsa staining. (c) Total number of CAFCs generated in the four groups after 7 and 14 days of expansion (), compared with the control group, compared with the MSC group. (d) Representative images of CAFCs. (e) Representative gel photograph of β-catenin protein expression.

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