Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage

Figure 2

Multilineage differentiation potential of unlabeled ASCs and labeled ASCs. The differentiation was induced 24 hours after labeling. The first and second rows show ASCs labeled with 5 μg/mL and 0.5 μg/mL H33342, respectively, after the differentiation procedures. In the last row, unlabeled ASCs after differentiation are shown. First column: oil red O staining after adipogenic differentiation. Second and third column: von Kossa staining (black nodules detecting phosphates) and alizarin red staining (detecting red calcium deposits) after osteogenic differentiation. Last column: alcian blue staining detects glycosaminoglycans (GAGs) of the extracellular matrix after chondrogenic differentiation. Scale bar represents 100 μm.