Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage

Table 1

Contamination of unlabeled ASCs by cocultured H33342 labeled ASCs (0.5 μg/mL and 5 μg/mL) in transwell plates after 2 days and 7 days. Two different washing procedures were performed. The occurrence of an undesired colabeling and the required exposition time for detection is indicated.

	2 days		7 days
Washing procedure	Labeled and 2x rinsed	Labeled, rinsed, detached, centrifuged and washed, cultivated for 2 days, and 2x rinsed	Labeled and 2x rinsed	Labeled, rinsed, detached, centrifuged and washed, cultivated for 2 days, and 2x rinsed
H33342	Detection of colabeled cells
0.5 µg/mL	No	No	No	No
5 µg/mL	Yes (200 ms)	Yes (200 ms)	Yes (200 ms)	Yes (200 ms)