Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures

Figure 1

Mononuclear cell infiltration in the synovial membrane and MSC differentiation results. (a) The boxplot diagram displays the percentages of positive cells for the mononuclear cell fraction, CD14+ monocytes, CD16+CD56+ NK cells, CD4+ and CD8+ T-cells, and B-cells in the synovial membrane of 10 patients enclosed in the study. (b–d) Differentiation into the three lineages was successful in all MSCs ( patients per assay), while important donor-dependent variations were observed. (b) The figure shows representative results of adipogenic differentiation at d14 and d21 as defined by Oil Red O-stained lipid vacuoles. (c) Osteogenic differentiation was determined by calcium deposition through Alizarin Red staining (quantitative analysis of the reextracted dye is depicted in the diagram) and was enhanced in BM-MSCs compared to SM-MSCs. (d) Chondrogenic differentiation was assessed by Safranin O and Collagen II staining. refers to significant differences between the groups ().

(a)

(b)

(c)

(d)