Research Article

Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures

Figure 3

Surface marker expression on CD4+ T-cells in monocultures and MSC-cocultures. (a) The diagrams display the mean positive cells for CD4+CD25+FoxP3+, CD4+CD25+CD127-, CD45R0+ Tregs, and CD45RA+ Tregs as detected by flow cytometry (). and . (b) Representative flow cytometry plots for background fluorescence, CD4+ T-cell monocultures at day 0 and day 5, and the respective BM-MSC and SM-MSC cocultures at day 5. Lymphocytes were gated for CD4, CD25, and FoxP3.