Characterization of Neurogenic Potential of Dental Pulp Stem Cells Cultured in Xeno/Serum-Free Condition: In Vitro and In Vivo Assessment

Figure 1

Strategy for derivation of neural stem-like cells from dental pulp of extracted human third molar. (a) Experimental scheme for classification of fetal bovine serum (FBS) and neural stem cell (NSC) groups. (b) Comparison of primary cell expansion and morphology between FBS and NSC medium cells. Scale bar = 100 μm. (c) MTT assay to measure cellular metabolic activities of FBS medium cells, NSC medium cells at passage 5, and human bone-marrow-derived mesenchymal stem cells (BM-MSCs). The error bars represent the standard deviation of mean from three independent experiments from one donor (FBS and NSC groups) or one cell line (BM). Two-way repeated-measures ANOVA (Greenhouse-Geisser) revealed significant effects of day, cell group, and day × cell group interaction (). The asterisk () indicates significant difference in comparison with BM-MSC at each time point according to one-way ANOVA and Games-Howell post hoc test ().

(a)

(b)

(c)