Naringin Stimulates Osteogenic Differentiation of Rat Bone Marrow Stromal Cells via Activation of the Notch Signaling Pathway

Table 1

PCR primer sequences and cycling conditions.

Gene and GenBank accession number	Primer sequence (forward/reverse)	Temperature (°C)	Product size (bp)
ALP (J03572)	5′-TCCGTGGGTCGGATTCCT-3′	58.0	280
	5′-GCCGGCCCAAGAGAGAA-3′
BSP (NM_012587)	5′-GCTATGAAGGCTACGAGGGTCAGGATTAT-3′	59.1	386
	5′-GGGTATGTTAGGGTGGTTAGCAATGGTGT-3′
Cbfa1 (AF053950)	5′-CCTCACAAACAACCACAGAAC CA-3′	60	325
	5′-AACTGA AAATACAAA CCATACCC-3′
PPARγ2 (NM_013124)	5′-ATCCCGTTCACAAGAGCTGA-3′	54.8	177
	5′-GCAGGCTCTACTTTGATCGC-3′
β-actin (NM_031144)	5′-ATCGTGGGCCGCCCTAGGCA-3′	61.0	260
	5′-TGGCCTTAGGGTTCAGAGGGG-3′

Note: ALP, alkaline phosphatase; BSP, bone sialoprotein; Cbfa1, core-binding factor a1; PPARγ2, peroxisome proliferator-activated receptor gamma 2; β-actin.