Gingival Mesenchymal Stem/Progenitor Cells: A Unique Tissue Engineering Gem

Table 1

Human G-MSCs isolation protocols.

Protocol number	Tissue culture method	Study
(I)	() Collected tissue incubated overnight with 2 mg/mL dispase at 4°C overnight to separate epithelium; () The minced tissues are digested in 4 mg/mL collagenase IV for 2 h at 37°C; (3) Cell filtered through 70 µm strainer; () Cells seeded out; () Single-cell cloning	[25]
(II)	() Tissue mincing; () Tissue digestion in 0.1% collagenase and 0.2% dispase for 15 min at 37°C; () Discarding of the first cell fraction containing some epithelial cells; () Tissues are that further incubated with enzyme solution for 5, 10, and 15 min and all cell fractions that are pooled; () Cells seeded out in tissue culture flasks	[18]
(III)	() Tissues digested with 0.4% dispase for 30 min at 37°C followed by collagenase type I (0.66 mg/mL) for 50 min; () Cell filtered through 70 µm strainer to single-cell suspensions; () Single-cell cloning	[20]
(IV)	() The minced tissues are digested in 3 mg/mL collagenase and 4 mg/mL dispase for 2 hours at 37°C; () Cell filtered through 70 µm strainer; () Single-cell suspension plated at a concentration of 60 cells/cm^2; () Selection of single-cell-derived colonies	[23]
(V)	() Tissue deepithelized under magnification and cut in small pieces (2 × 2 mm) and rinsed; () Tissue placed in dry culture flasks to adhere for 30 min then medium slowly added; () Flasks incubated for cells to grow out; () STRO-1 magnetic cell sorting	[19]
(VI)	() The minced tissues are digested in 2 mg/mL collagenase and 1 mg/mL dispase for 30 min; () Discarding of the first cell fraction containing some epithelial cells; () Tissues that are further incubated with same enzyme solution for 90 min at 37°C; () Cell filtered through 70 µm strainer; () Cells seeded out	[24]