Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation

Figure 6

Effect of PL on osteogenic differentiation of DPSCs. Alizarin red staining on undifferentiated sample cultured in growth medium with 10% FBS (a) or 1% PL (d) and on samples differentiated with osteogenic medium containing 20% FBS (b) or 1% PL (e). Relative quantification of the same staining by spectrophotometry. Data shown as mean + SD, samples. Two different experiments were performed. Significant levels according to unpaired -test (; ; ; ). (g) Relative quantification of osteogenic markers by real-time PCR on samples derived from the end (28 days) of the differentiating protocol. Data shown as mean + SD, . Significant levels according to unpaired -test (; ; ; ).