Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching

Figure 2

(a) Microphotographs of LIVE/DEAD® staining of seeded scaffold samples (viable cells shown in green, dead cells in red; left), hematoxylin and eosin staining of paraffin sections (middle), and procollagen 3A1 immunostaining of paraffin sections (positive staining in dark brown; right), 24 h after mechanical stimulation. (b) Score points for cell viability (left) or cell morphology, alignment, and distribution on the scaffold surface (L/D score; middle), obtained by evaluation of LIVE/DEAD stained samples, the horizontal lines representing the monolayer controls, and score points for cell integration and cell layer integrity (HE score, right), obtained by evaluation of hematoxylin and eosin stained sections; bars represent the median values and error bars the 95% confidence interval; marks significant differences compared to the monolayer control (); # means significant differences between the sample groups indicated (); stat: static; stim: mechanical stimulation.

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