Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching

Table 1

Histology score systems. Score systems used for evaluation of LIVE/DEAD stained samples (viability score and L/D score) and hematoxylin and eosin stained sections (HE score).

(a)

Viability score
Description	Score points
<10% vital cells	0
10–50% vital cells	1
>50–75% vital cells	2
>75% vital cells	3

(b)

L/D score
Cell morphology		Cell alignment		Cell distribution
Description	Score points	Description	Score points	Description	Score points
Predominantly rounded	0	Predominantly random	0	Focal	0
Predominantly polygonal or equal numbers rounded and elongated	1	Equal numbers random and parallel	1	Multifocal	1
Predominantly elongated	2	Predominantly parallel	2	Homogeneous	2

(c)

HE score
Cell integration		Cell layer integrity
Description	Score points	Description	Score points
None	0	Single cells only	0
Integration of single cells	1	Nonhomogeneous cell layer	1
Integration of >50% of cells	2	Homogeneous cell layer	2