Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects

Figure 4

Relative expressions of PSC markers, Oct-4, Nanog, and Sox2, were measured by RT-qPCR and compared using equal amounts of mRNA isolated from CNT PB of young, middle, and older healthy volunteers. The relative expression of each PSC marker is calculated according to a positive control PCR (RNA isolated from H9 and HUES 3 hESC lines). Data represent the mean ± standard deviation for each age group. The difference in mRNA expression of these markers between the three groups of age was not statistically significant (, Kruskal-Wallis test).