Hepatic Stellate Cell-Derived Microvesicles Prevent Hepatocytes from Injury Induced by APAP/H₂O₂

Figure 3

Effect of HSC-MVs on APAP or H₂O₂-treated BRL-3A and HL-7702 apoptosis and cleaved caspase-3 expression. (a) BRL-3A apoptosis monitored by Hoechst 33258 staining (red arrows represent apoptotic cells, green arrows represent normal cells). (b) BRL-3A apoptosis determined by flow cytometric analysis. (c) Expression of cleaved caspase-3 protein in BRL-3A. HL-7702 apoptosis detected by Hoechst 33258 staining (d) and flow cytometric analysis (e). (f) Expression of cleaved caspase-3 protein in HL-7702. versus vehicle, APAP; versus vehicle, H₂O₂; ^#, ^## versus treatment control, APAP; , versus treatment control, H₂O₂; versus L-HSC-MVs, APAP; versus L-HSC-MVs, H₂O₂; , versus M-HSC-MVs, APAP; versus M-HSC-MVs, ; /group; ~ represents < 0.05 versus L-HSC-MVs, APAP; & represents < 0.05 versus L-HSC-MVs, H₂O₂.

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