Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children

Figure 3

Verification of microarray results by immunohistochemical (IHC) staining. Hematoxylin-eosin staining in the gingiva (a, f) and dental follicles (DFs) (k, p) (asterisk: outer border neighboring alveolar bone). IHC staining for CXCL10 in the gingiva (b, g) and DFs (l, q). IHC staining for CSTA in the gingiva (c, h) and DFs (m, r). The expression of CXCL10 and CSTA was stained markedly in the gingival epithelium. The IHC staining for AMBN in the gingiva (d, i) and DFs (n, s). AMBN was stained around the outer layer of the DFs. The IHC staining for CXCL12 in the gingiva (e, j) and dental follicles (o, t). CXCL12 was stained in both a cellular layer and the collagenous connective tissue of DFs (scale bars: 200 μm).