Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Figure 1

Schematic overview of the protocol used for the hepatic differentiation. The DE cells were harvested at day 7 and replated in HEP progenitor medium (2) on plates coated with the specific hepatocyte coating (1), which is included in the prototype of Cellartis Hep differentiation kit (Takara Clontech). Medium change with HEP progenitor medium (2) was performed at days 9 and 11. At days 14 and 16 the cells received HEP maturation medium base (3Ap) and supplement (3Bp). At day 18 the cells received HEP maturation medium base (3Ap). At days 21, 23, 25, 28, 30, and 32 the cells received HEP maintenance medium (4); thereafter they were maintained in culture until day 35.