Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Figure 2

Morphology of cells during hepatic differentiation at days 0, 5, 7, 9 (ChiPSC6b), 11 (P11012), 14 (ChiPSC6b), 16 (P11012), 18 (P11012), 21 (P11012), 24 (SA121), 29 (AS034), and 35 (ChiPSC6b) magnification 10x. (a) Human PSCs cultured in Cellartis DEF-CS demonstrate stem cell morphology at day 0 (AS034). (b) At day 5 (P11025) and day 7 (SA181) the cells show a characteristic DE morphology. (c) Following the application of HEP progenitor medium, the DE cells transferred gradually to hepatoblasts. (d) The cells acquired the hepatocyte morphology (polygonal cells) following the application of HEP maturation medium (3Ap) and supplement (3Bp). In the HEP maintenance medium (4) the cells preserve typical hepatocyte morphology, polygonal cells with single- or binucleus.

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