Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Figure 7

Gene expression profiles of later hepatic markers (AAT, PROX1, KRT18, ALB, and CYP3A4) in the interval from day 13 to day 35. (a) The -axis indicates the days during differentiation and the -axis indicates RQ, where the calibrator’s RQ = 1. The colored lines show the results from each individual hPSC line and the grey line indicates the calculated median value of the RNA level. Correlation tables are shown in (b). The graphs show synchronicity of onset and up- and downregulation among all cell lines for the genes AAT, PROX1, KRT18, ALB, and CYP3A4. The average correlation between pairs of cell lines ranges from weak (0.38 for CYP3A4) to strong (0.75 for KRT18 and ALB).