Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Table 2

Summary of primary and secondary antibodies used in the study.

Primary antibody	Class	Dilution	Distributor,; catalogue number	Secondary antibody	Dilution	Distributor, catalogue number	Applied to cells day
Albumin (ALB)	Rabbit-IgG	1 : 500	DakoCytomation, A0001	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	24, 25, 29, 30
CYP3A (4)	Rabbit-IgG	1 : 200	Cypex, PAP011	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	29, 31
CK 18	Mouse-IgG1	1 : 100	DakoCytomation, M7010	Goat anti-mouse-IgG-Alexa 488	1 : 500	ThermoFisher Scientific, A-11029	24, 25, 29, 30
HNF4a	Rabbit-IgG	1 : 400	Santa Cruz, sc-8987	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	14, 24, 25, 29, 30
Oct4	Mouse-IgG2b	1 : 200	Santa Cruz, sc-5279	Donkey anti-mouse-IgG-Alexa-488	1 : 1000	ThermoFisher Scientific, A-21202	0, 5, 7
Sox17	Goat-IgG	1 : 500	R&D Systems, AF1924	Donkey anti-goat-IgG Alexa 594	1 : 1000	ThermoFisher Scientific, A-11058	5, 7
SSEA-1	Mouse-IgM	1 : 200	Santa Cruz, sc-21702	Donkey anti-mouse-IgG-Alexa-488	1 : 1000	ThermoFisher Scientific, A-21202	0
AAT	Rabbit-IgG	1 : 200	DakoCytomation, A0012	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	24, 25, 29, 30
α-fetoprotein (AFP)	Mouse-IgG1	1 : 500	Santa Cruz, sc-51506	Goat anti-mouse-IgG-Alexa 594	1 : 1000	ThermoFisher Scientific, A-11032	14, 24, 25, 29, 30
BSEP	Goat-IgG	1 : 100	Santa Cruz, sc-17292	Donkey anti-goat-IgG-Alexa-488	1 : 1000	ThermoFisher Scientific, A-11055	29
OATP-C (OATP1B1)	Rabbit-IgG	1 : 200	Santa Cruz, sc-33609	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	29
NTCP	Goat-IgG	1 : 400	Santa Cruz, sc-107030	Donkey anti-goat-IgG-Alexa-594	1 : 1000	ThermoFisher Scientific, A-11058	29
MRP2	Rabbit-IgG	1 : 50	Santa Cruz, sc-20766	Donkey anti-rabbit-IgG-Alexa 488	1 : 1000	ThermoFisher Scientific, A-21206	29