Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold

Figure 1

Clinical techniques for harvesting alveolar bone marrow. (a) The cancellous bone was drilled at 50 rpm until achieving the desired diameter and length for the implant site. (b) Before dental implant placement, bone marrow was harvested using a flapless, minimally invasive surgical procedure. (c, d) Bone marrow scraped from the pilot drill was immediately placed in sterile tubes and transported to the laboratory. (e) Confirmation of the initial stability of the dental implant by cone-beam CT. (f) Photomicrograph of aBMSCs 7–10 days following initial plating, revealing a fibroblastic, spindle shaped morphology similar to that of stem cells from other cell sources. Magnification: ×400.

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