Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants

Figure 1

The Wnt receptor frizzled-4 is expressed in neural cells in all layers of the gut wall. Immunohistochemical analysis for frizzled-4 (Fzd4), , and nestin expression on cryostat sections of human colon (sample number 1). ((a1)–(a3)) Overview of combined (green) and Fzd4 (red) immunostaining; (a1); Fzd4 (a2); merge (a3); DAPI (blue); tunica muscularis (TM); tela submucosa (TSM); scale bar: 200 μm. ((b1)–(b3)) Higher magnification of combined (green) and Fzd4 (red) immunostaining of myenteric ganglia; (b1); Fzd4 (b2); merge (b3); DAPI (blue); scale bar: 50 μm. ((c1)–(c3)) Combined nestin (green) and Fzd4 (red) immunostaining of myenteric ganglia; nestin (c1); Fzd4 (c2); merge (c3); DAPI (blue); scale bar: 50 μm.