Effects of Human Umbilical Cord Mesenchymal Stem Cells on Human Trophoblast Cell Functions In Vitro

Figure 5

Effect of hUCMSCs and hFFs on the migration ability of HTR-8/SVneo trophoblast cells. (a) HTR-8/SVneo cells (2 × 10⁴ cells/well) were cultured in medium containing 0, 25, 50, or 100% hUCMSC (a1–a4) or hFF (b1–b4) supernatant in Transwell inserts for 16 hours or were cocultured with hUCMSCs (c1–c4) or hFFs (d1–d4) at a concentration of 0, 2.5, 5, or 10 × 10⁴ cells/well in Transwell inserts for 16 hours. Cells that had migrated were stained with calcein-AM. (b) HTR-8/SVneo cells that had migrated were counted after staining. represents significant differences versus control (); represents very significant differences versus control (); # represents significant differences between hUCMSCs and hFFs at an individual dose ();  ## represents very significant differences between hUCMSCs and hFFs at an individual dose ().