Effects of Human Umbilical Cord Mesenchymal Stem Cells on Human Trophoblast Cell Functions In Vitro

Figure 8

Effect of hUCMSCs and hFFs on cytokine secretion of HTR-8/SVneo cells as detected by real-time PCR. (a–c) HTR-8/SVneo cells (10 × 10⁴ cells/well) were cultured in 0, 25, 50, or 100% hUCMSC or hFF supernatant in medium for 48 hours. (d–f) HTR-8/SVneo cells (10 × 10⁴ cells/well) were cocultured with hUCMSCs or hFFs at a concentration of 0, 2.5, 5, or 10 × 10⁴ cells/well in 6-well Transwell plates for 48 hours. Cytokine amounts were assessed in the resulting culture medium. represents significant differences versus control (); represents very significant differences versus control (); # represents significant differences between hUCMSCs and hFFs at an individual dose ().