Priming Adipose-Derived Mesenchymal Stem Cells with Hyaluronan Alters Growth Kinetics and Increases Attachment to Articular Cartilage

Figure 1

MSCs grown in control media and seeded in HA media. MSCs expanded until passage 2 in control media. Cells from flask (A) were then stripped and cell suspensions were counted using the standard enumeration technique. Cells were seeded into 96-well plates in either control media (control) (−−) or HA media (− +) containing a series of concentrations ranging from 0.25 to 2 mg/mL of HA to make up the five conditions (-axis). Conditions were tested using five technical replicates per 96-well plate and run in biological triplicate (). Wells were assayed at the endpoint using Cell Counting Kit-8 with standard curves run on every 96-well plate seeded 24 hours prior to endpoint and absorbance read at a wavelength of 450 nm (-axis). All conditions were compared back to the control using a -test ( value = 0.07, value < 0.05). (a) MSCs seeded in standard 96-well plates for 24 hours (adherence), (b) MSCs seeded in standard 96-well plates for three days (proliferation), (c) MSCs seeded in high-adherence 96-well plates for 24 hours (adherence), and (d) MSCs seeded in high-adherence 96-well plates for three days (proliferation) (see Supplementary Table 1).

(a)

(b)

(c)

(d)