Characterization of Cellular and Molecular Heterogeneity of Bone Marrow Stromal Cells
Table 3
Whole genome microarray analysis of two clonal populations of human bone marrow stromal stem cells: CL1 and CL2 cells. (a) Top 35 highly upregulated genes in CL1 cells versus CL2 cells. (b) Ingenuity® Pathway Analysis (IPA®) showing the different physiological system development and function genes found in each category and the corresponding value. (c) Upper part IPA analysis showing the categories for skeletal and muscular system development and function upregulated in CL1 cells and lower part showing genes upregulated in CL2 cells. NOV = nephroblastoma overexpressed, IGFBP5 = insulin-like growth factor binding protein 5, ALP, TAGLN = transgelin, and CDH11 = OB-cadherin (osteoblast).
(a)
Gene ID
Fold change CL1 versus CL2
FOLR3
28.4721
CCL3L3
17.936
POSTN
15.5924
SERPINB2
−17.2599
IGFBP5
14.6708
CCL3
13.1203
NOV
11.2921
ACTG2
10.4493
CRYAB
10.0678
PSG4
9.68913
RAB3IL1
9.16897
SCIN
9.13702
MYL9
9.12814
TNFRSF11B
8.86049
TAGLN
8.75581
CDH12
8.06682
SHISA2
8.0291
THBS1
7.86854
SPP1
7.64205
LCE2A
7.41042
TMEM98
7.38011
PSG7
7.12183
MYPN
7.01837
FNDC1
6.88102
TNS3
6.72083
ABI3BP
6.67822
LRP3
6.64307
MMP3
6.34715
FAM167A
6.02684
HSPB2
6.01063
ALPL
6.01022
CTSK
5.87356
CXCL12
5.68572
THY1
4.89445
CDH10
4.86105
(b)
Name
value
# molecules
Physiological system development and function
Organismal development
181
Embryonic development
154
Organ development
145
Skeletal and muscular system development and function
123
Tissue development
236
(c)
Functions annotation
value
# molecules
Skeletal and muscular system development and function upregulated in CL1 cells
Size of bone
24
Differentiation of osteoblasts
25
Mineralization of bone
19
Bone mineral density
19
Skeletal and muscular system development and function upregulated in CL2 cells
