Skeletal Muscle-Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury

Figure 4

Multilineage differentiation of MDSPCs and BM-MSCs. Cells cultured in osteogenic differentiation medium formed bone nodules as determined by von Kossa staining. Cells cultured in chondrogenic differentiation medium had lacunae surrounded by the Alcian blue-stained matrix. Cells cultured in adipogenic medium formed lipid droplets, which were positive for oil red O staining. BM-MSCs and MDSPCs differentiated into myogenic lineage and became multinucleated, elongated, and highly positive for desmin (>98%).