Skeletal Muscle-Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury

Table 1

Primer sequences and conditions for real time reverse transcription polymerase chain reaction.

Gene	Primer nucleotide sequence	Product size (bp)	Annealing temperature (°C)
β-actin	5′-GCACMATGAAGATCAAGATCATTGCTCC-3′ (forward)	118	60
	5′-TCRTACTCCTGCTTGCTGATCCAC-3′ (reverse)
Oct4	5′-GGCCCCTGCTGGAGAAGTG-3′ (forward)	120	60
	5′-CACGGTTCTCAATGCTAGTCCGC-3′ (reverse)
Sox2	5′-TCAGCGCCCTGCAGTACAAC-3′ (forward)	140	60
	5′-GGCCTCGGACTTGACCACAG-3′ (reverse)
NANOG	5′-GGTTGAAGACTAGCAACGGTCTGACT-3′ (forward)	81	60
	5′-AGCCCTGAGAATAGCTGCAATGG-3′ (reverse)