The Effects of High Glucose on Adipogenic and Osteogenic Differentiation of Gestational Tissue-Derived MSCs

Figure 5

The effect of high glucose on osteogenic differentiation of BM-MSCs, CH-MSCs, PL-MSCs, and UC-MSCs. (a) Graph shows relative mRNA levels of osteogenic genes RUNX2, OSX, and OCN of BM-MSCs, CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 7 (). (b) Graph shows relative mRNA levels of osteogenic genes RUNX2, OSX, and OCN of BM-MSCs, CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 14 (). (c) Representative micrographs show the morphology of osteocyte-like cells (purple colored cells) derived from BM-MSCs, CH-MSCs, PL-MSCs, and UC-MSCs cultured in osteogenic differentiation medium with or without glucose supplementation for 14 days after being subjected to BCIP/NBT alkaline phosphatase activity assay. Scale bar: 100 μm. Data were presented as mean ± standard error of the mean (SEM). versus OSTEO. corresponds to the number of independent samples used in the experiments. DMEM: MSCs cultured in complete medium which serve as nondifferentiation controls. OSTEO: MSCs cultured in osteogenic differentiation medium without glucose supplementation which serve as normal glucose controls. OSTEO + glucose: MSCs cultured in osteogenic differentiation medium supplemented with 25 mM D-glucose.