The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45^lowCD271⁺ Phenotype

Figure 3

BM MSC enumeration and gene expression using CD45^lowCD271⁺ phenotype. (a) Correlation between MSCs identified as CD45^lowCD271⁺ cells with donor age across complete age range (total 67 donors, left), between three age groups (centre), and between CFU-F/ml and donor-matched CD45^lowCD271⁺ cells/ml (right). Black lines across data sets indicate line of best fit (left and right) and median values for each group (centre). (b) Expression of transcripts associated with osteogenic (top) and adipogenic differentiation (bottom). (c) Expression of transcripts associated with intercellular communication. (d) Expression of transcripts associated with bone remodelling. Black and grey lines indicate median values with interquartile range in young and old donors, respectively. , ; young donors (19-38 years old) and 8 old donors (61-89 years old). CD271: CD45^lowCD271⁺ MSCs; HLC: CD45^highCD271^- hematopoietic lineage cells.

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