Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists

Figure 3

Flow cytometry analysis of the expression of cell surface markers in rBM-MSCs. Preconditioning with polyinosinic-polycytidylic acid (Poly(I:C)) or lipopolysaccharide (LPS) did not change the phenotype of rat bone marrow-derived mesenchymal stromal cells (rBM-MSCs). (a) Representative immunophenotype data for rBM-MSCs 23 h after a brief stimulation (1 h) with LPS (MSC-LPS) or Poly(I:C) (MSC-Poly(I:C), compared to unstimulated cells (MSC). Histograms show cells stained with isotype-matched control antibodies in gray and cells stained with antibodies of interest in color. On the left, dot plot representations showing the distribution of cell populations. FSC: forward scatter; SSC: side scatter. (b) Quantitative analysis showing that rBM-MSCs expressed CD90 and CD29 (≥98% of the cells), whereas few expressed CD34 and CD45 (expressed by ≤4.12% of the cells) in all conditions ( biological replicates). Graphs show individual values and the .

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