Mesenchymal Stromal Cells Are More Effective Than Their Extracellular Vesicles at Reducing Lung Injury Regardless of Acute Respiratory Distress Syndrome Etiology

Table 1

Lung morphometry.

Groups		Normal alveoli (%)	Alveolar collapse (%)	Neutrophils (%)	Interstitial edema	Collagen fibers (%)
Cp
ARDSp	SAL
	MSC
	MSC serum
	EV	^,#	^,#	^,#
	EV serum	^,†	^,†	^,†
Cexp
ARDSexp	SAL
	MSC
	MSC serum
	EV	^,#	^,#	^,#
	EV serum	^,†	^,†	^,†

Fraction area of normal and collapsed alveoli, neutrophil cell count, interstitial edema, and collagen fiber content in the alveolar septa. All values were computed in 10 random, noncoincident fields of view per mouse. Values were expressed as deviation of six animals per group. ARDS was induced by administration of Escherichia coli lipopolysaccharide intratracheally (ARDSp) or intraperitoneally (ARDSexp). Control mice (C) received saline solution intratracheally (Cp) or intraperitoneally (Cexp). After 24 h, ARDSp and ARDSexp animals were further randomized to receive saline (50 μL, SAL), bone marrow-derived MSCs (10⁵, 50 μL), or EVs (10⁵, 50 μL), stimulated (MSC serum, EV serum) or not with serum (MSCs, EVs) obtained from ARDSp or ARDSexp animals. Significantly different from the corresponding C group. Significantly different from the corresponding ARDS group. ^#Significantly different from MSC. ^†Significantly different from MSC serum ().