Shikonin Induces Glioma Necroptosis, Stemness Decline, and Impedes (Immuno)Proteasome Activity

Figure 2

Shikonin predominantly promoted U87 and T98G stem-like cells death. Images of annexin V/PI doubling staining of (a) U87 stem-like cells and (b) T98G stem-like cells treated with increasing concentrations of Shikonin (0, 4, 8, and 12 μM) for 48 hr and then subjected to flow cytometry. The apoptotic cell ratios of (c) U87 stem-like cells (Annexin V⁺/PI⁺) and (d) T98G stem-like cells (Annexin V⁺/PI⁺ and Annexin V⁺/PI⁻) were calculated. Data are expressed as mean ± SEMs (n = 5 per group). ()() versus untreated cells.

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