Shikonin Induces Glioma Necroptosis, Stemness Decline, and Impedes (Immuno)Proteasome Activity

Figure 6

Shikonin reduced proteasome activities. U251, U87, and T98G cells were treated with increasing concentrations of Shikonin (0, 4, 8, and 12 μM) for 24 hr. Caspase-like, trypsin-like, and chymotrypsin-like proteasome activities of (a) U251, (b) U87, and (c) T98G cells were measured by fluorogenic substrates. Data are expressed as mean ± SEMs (n = 3 per group). ()() versus untreated cells.

(a)

(b)

(c)