Shikonin Induces Glioma Necroptosis, Stemness Decline, and Impedes (Immuno)Proteasome Activity

Figure 7

Heatmap showing transcriptomic sequencing results of the effect of Shikonin on the expression levels of ubiquitinated proteins and proteasome subunits. U251 cells were treated with Shikonin for 24 hr; Shikonin decreased the expression levels of immunoproteasome catalytic subunits and increased ubiquited proteins. The green box highlights that the expression of the constitutive proteasome subunits PSMB1, PSMB2, and PSMB5 was increased, while the expression of the immunoproteasome subunits PSMB8, PSMB9, PSMB10, PSME1, PSME2, and PSME3 was decreased.