METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 1

Cell identification and METTL3 promoted osteogenic differentiation of hPDLSCs and pPDLSCs: (a) surface makers of hPDLSCs and pPDLSCs were identified by flow cytometry; (b) clone formation assays of hPDLSCs and pPDLSCs; (c) the quantitative analysis diagram for clone formation assays; (d) cell proliferation assays of hPDLSCs and pPDLSCs; (e) the expressions of methyltransferase (METTL3 & METTL14) and demethylases (ALKBH5 and FTO) in hPDLSCs and pPDLSCs; (f) the protein levels of METTL3 in hPDLSCs and pPDLSCs; (g and h) transfection efficiency of METTL3 was detected by qRT-PCR and western blotting; (i) osteogenic differentiation was detected by ARS staining and ALP staining at 21 and 7 days of osteogenic induction after METTL3 knockdown; (j) the mRNA levels of ALP, Runx2, and Col1 were detected at 7 days of osteogenic induction after METTL3 knockdown; (k) the protein level of Runx2 was determined by western blotting at 7 days of osteogenic induction after METTL3 knockdown; (l) osteogenic differentiation was detected by ARS staining and ALP staining at 21 and 7 days of osteogenic induction after METTL3 overexpression; (m) the mRNA levels of ALP, Runx2, and Col1 were detected at 7 days of osteogenic induction after METTL3 overexpression; (n) the protein level of Runx2 was determined by western blotting at 7 days of osteogenic induction after METTL3 overexpression. Significance levels were determined by () , () , () , and () .

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