METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 2

METTL3 promoted osteogenic differentiation by promoting the expression and stability of lncRNA CUTALP: (a) MeRIP-qPCR was used to detect the six m6A-modified lncRNAs; (b) the expression levels of lncRNA CUTALP in hPDLSCs and pPDLSCs were detected by qRT-PCR; (c and d) the expression and methylation modification levels of lncRNA CUTALP after METTL3 overexpression and knockdown in pPDLSCs were detected by qRT-PCR and MeRIP-qPCR; (e) the RNA decay rate assay was detected the half-life of lncRNA CUTALP after METTL3 knockdown; (f) the RIP-qPCR was carried to confirm the binding between METTL3 and lncRNA CUTALP; (g and h) qRT-PCR and western blotting showed that the inhibition of osteogenic markers caused by METTL3 knockdown in pPDLSCs would be recovered by lncRNA CUTALP overexpression. Significance levels were determined by () , () , () , and () .

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