METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 3

LncRNA CUTALP promoted osteogenic differentiation of hPDLSCs and pPDLSCs: (a) transfection efficiency of lncRNA CUTALP was detected by qRT-PCR; (b) osteogenic differentiation was detected by ARS staining and ALP staining at 21 and 7 days of osteogenic induction after lncRNA CUTALP knockdown; (c) the protein level of Runx2 was determined by western blotting at 7 days of osteogenic induction after lncRNA CUTALP knockdown; (d) the mRNA levels of ALP, Runx2, and Col1 were detected at 7 days of osteogenic induction after lncRNA CUTALP knockdown; (e) osteogenic differentiation was detected by ARS staining and ALP staining at 21 and 7 days of osteogenic induction after lncRNA CUTALP overexpression; (f) the mRNA levels of ALP, Runx2, and Col1 were detected at 7 days of osteogenic induction after lncRNA CUTALP overexpression; (g) the protein level of Runx2 was determined by western blotting at 7 days of osteogenic induction after lncRNA CUTALP overexpression; (h) qRT-PCR was used to detect the expression changes of lncRNA CUTALP with osteogenic induction; (i) correlation analysis between lncRNA CUTALP levels and Runx2 levels in pPDLSCs 0, 1, 7, and 14 days after osteogenic induction. Significance levels were determined by () , () , () , and () .

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