METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients

Figure 4

LncRNA CUTALP regulated the osteogenic differentiation of pPDLSCs via competitively binding miR-30b-3p: (a) schematic diagram and sequences of miR-30b-3p predicted target site in lncRNA CUTALP; (b) dual-luciferase reporter assays were performed to measure the luciferase activity in 293T cells; (c) qRT-PCR was used to detect the expression level of miR-30b-3p after lncRNA CUTALP knockdown and overexpression; (d) transfection efficiency of miR-30b-3p mimic and inhibitor was detected by qRT-PCR; (e) the expression levels of lncRNA CUTALP after transfection with miR-30b-3p mimic and inhibitor were detected by qRT-PCR; (f) correlation analysis between miR-30b-3p levels and lncRNA CUTALP levels in pPDLSCs 0, 1, 7, and 14 days after osteogenic induction. Significance levels were determined by () , () , () , and () .

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